Database : HANSEN
Search on : MYCOBACTERIUM TUBERCULOSIS [Subject descriptor]
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Id:19398
Author:Roche, Paul W; Neupane, Kapil Dev; Failbus, Sarah S; Kamath, Arun; Britton, Warwick J.
Title:Vaccination with DNA of the Mycobacterium tuberculosis 85B antigen protects mouse foot pad against infection with M. leprae.
Source:Int. J. Lep;69(2):93-98, Jun., 2001. tab, graf.
Abstract:A DNA vaccine composed of the gene for the common mycobacterial secreted protein antigen 85B was demonstrated to protect the mouse foot pad against infection with Mycobacterium leprae. The protective effect was demonstrated by a 61%-88% reduction in the bacterial number, a protective effect less than that of BCG. The same DNA vaccine has been shown to protect mice against M. tuberculosis infection, and the importance of testing other candidate tuberculosis vaccines for their potential to protect against leprosy is discussed.(AU)^ien.
Descriptors:Mycobacterium tuberculosis/imunol
DNA/genet
DNA/imunol
Mycobacterium leprae/imunol
Limits:Humanos
Location:BR191.1


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Id:19143
Author:Hadler, W. A; Zitti, L. M.
Title:Estudo da sensibilidade tubercul¡nica em cobaios normais inoculados experimentalmente com M. leprae, M. lepraemurium e M. tuberculosis / Study of tuberculin sensitivity in normal guinea pigs inoculated with M. leprae, M. lepraemurium and M. tuberculosis
Source:Rev. bras. Leprol;21(4):341-364, dez. 1953. ^btab, ^bgraf.
Abstract:The tuberculin sensitivity of 173 guinea-pigs was determined by testing with o,05 ml of 1/10 O.T. These animals were inculated with known doses of M. leprae or M. lepraemurium and the results were compared with reaction obtained in inoculated BCG., an in normal non-inoculated (control) guinea-pigs. The bacillary contents of the M. leprae and M. lepraemurium suspension were estimated by weighing the bacilli contained in 10-20 ml of the suspension, after chlorofrom separation. The guinea-pigs were divided in four groups: Group 1 - 59 guines-pigs inoculated with M. leprae suspensions as follows: a - 10 inoculated with suspension containing nearly 10.0 mg of bacilli by peritoneal route; b - 20 inoculated with suspension containing nearly 0.8 mg of bacilli by peritoneal route; c - 10 inoculated with suspension containing nearly 0.17 mg of bacilli by intra-cutaneous route; d - 19 inoculated with "lepromin" containing 0.074 mg of bacilli (in 0.1 ml) by intra-cutaneous route. Group 2 - 38 guinea-pigs inoculated with M. lepraemurium suspension as follows: a - 38 inoculated with suspensions containing nearly 10.0 mg of bacilli by peritoneal route; b - 18 inoculated with suspensions containing nearly 3.33 mg of bacilli by peritoneal route. Group 3 - 51 guinea-pigs inoculated with BCG. suspensions as follows: a - 20 inoculated with suspension containing 10.0 mg of bacilli by peritoneal route; b - 31 inoculated with suspension containing 40.0 mg of bacilly by peritoneal route. Group 4 - 25 normal‡, noninoculated, guinea-pigs (control-group). After inoculation tuberculin reaction was made periodically by intracutaneous injections of 0.05 ml of 1/10 O.T. The results show taht the inoculation of 0.8 mg and 10.0 mg of M. leprae, by peritoneal route, suscitated tuberculin hypersensitivity in guinea-pigs, which presents the following characteristics: 1 - slow development attaining is maximun 30 days after inoculation; 2 - weak tuberculin reactions in 60.0-65.0 per cent of the...(AU)^ien.
Descriptors:Mycobacterium leprae/imunol
Mycobacterium lepraemurium/imunol
Mycobacterium tuberculosis/imunol
Limits:Humanos
Electronic Medium:http://hansen.bvs.ilsl.br/textoc/revistas/1953/PDF/v21n4/v21n4a06.pdf / pt
Location:BR191.1


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Id:19142
Author:Hadler, W. A.
Title:Estudo comparado das lesões provocadas pela injeção intradérmica de suspensões de M. leprae e M. tuberculosis em cobaios normais / Comparative study of injuries caused by intradermal injection of suspensions of M. leprae and M. tuberculosis in normal guinea pigs
Source:Rev. bras. Leprol;21(4):315-340, dez. 1953. ^bilus, ^btab, ^bgraf.
Abstract:The study of the tissue reactions suscitated by M. leprae and M. tuberculosis and the fate of inoculated bacterial cells, were made in normal guinea-pigs injected intracutaneously. 92 normal and tuberculin negative guinea-pigs were divided in 4 lots: 1) 32 injected with 0.1 ml of a suspension containing 0.33 mg of BCG.; 2) 20 injected with 0.1 ml of "lepromin" containing nearly 0.074 mg of M. leprae; 3) 20 injected with 0.1 ml of "lepromin" containing nearly 0.34 mg of M. leprae; 4) 20 injected with 0.1 ml. o a suspension containing 0.33 mg of BCG. one one side and 0.1 ml of "lepromin" containing nearly 0.34 mg of M. leprae on the other side. The cutaneous reation was observed macroscopically in the animals of the lots 1, 2 and 3; microscopic examination was in 60 guinea-pigs of the 4 lots. The macroscopic results shows: a) BCG. injection produce stronger inicially reaction, which presents total involution 24 days after inoculation; b) the "lepromin" (containing 0.34 mg of bacilli) produce weaker initial reaction, but the involution is slower, disappearing only 30-40 days after inoculation. Histologically both Mycobacteria suscitate reactions of the same type and with the same cytological constitution; there are only small quantitative differences. But the histological evolution of the lesions shows definite differences; the BCG. suscitate lesions which presents less slow evolution and reamin early without bacilli. In normal guinea-pigs injected intracutaneously with "lepromin", the M. leprae is phagocytozed and lyzed by macrophages; the substances liberated by lysis induced the transformation of the macrophage into "epithelioid cells". This latter to be endowed of high metabolic activity and produces splitting of substances liberated by bacillary lysis. The normal guinea-pigs tissue reactions against M. leprae (excluded the initial acute phasis) can be divided into two phasis: 1- phasis of lysis of bacilli, or macrophages phasis; 2 - phasis of splitting and ...(AU)^ien.
Descriptors:Hanseníase/fisiopatol
Mycobacterium leprae/imunol
Mycobacterium tuberculosis/imunol
Limits:Humanos
Electronic Medium:http://hansen.bvs.ilsl.br/textoc/revistas/1953/PDF/v21n4/v21n4a05.pdf / pt
Location:BR191.1


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Id:19054
Author:Passos, A. C. de; Baptista, Luis; Martins, A. B. C. Nogueira; Sampaio, B. Pedral; Silveira, Homero; Marques, Armando Ribeiro.
Title:O comportamento da reação de Mitsuda em tuberculosos após becegeização oral / The behavior of the Mitsuda reaction in tuberculosis patients after oral BCG
Source:Rev. bras. Leprol;20(1):1-19, mar. 1952. ^btab, ^bgraf.
Abstract:Os AA. iniciam seu trabalho fazendo uma revisão bibliogr fica sôbre a cos-sensibilização aos Mycobacterium tuberculosis e Mycobacterium leprae. Em seguida, falam da experiência brasileira com BCG por via digestiva, procurando demonstrar através de citações, fatos que comprovam a dissociação de alergia e imunidade. Referem à experiência de um dos autores usando a orobecegeização em portadores de dermatoses e hiperérgicos à tuberculina, nos quais observou o desaparecimento de lesões cutâneas e melhoria do estado geral, fatos interpretados como consequentes a um esforço de imunidade e dessensibilização. Procuram interpretar os casos de lepromatosos que apresentam reação de Mantoux positiva como sendo indiv¡duos que tiveram infecção tuberculosa, alergizaram-se e permaneceram alérgicos, mas perderam a imunidade de maneira a permitir a sôbre-existência da infecção leprótica. Passando ao estudo da reação da lepromona em tuberculosos, referem-se às experiências de outros e à sua no presente trabalho, depois de reveram a literatura sôbre a tolerância do BCG. O material de estudo constou de 57 doentes portadores de tuberculose pulmonar ativa, em graus diversos de evolução, todos internados no Hospital Clemente Ferreira, da Liga Paulista Contra a Tuberculose. Foi feita a reação de Mantoux a 1:10.000 (0,001 mg de tuberculina) e a 1:1.000 (0,01 mg de tuberculina), tendo encontrado 38 analérgicos e em 6 não puderam fazer a reação à tuberculina. A percentagem de tuberculina positiva foi de 74,5 por cento. Após a reação de Mantoux fizeram a de Mitsuda e, em 30 dias, verificaram em 33 doentes pápulas de diâmetro iguais ou maiores de 5 mm; em 22, pápulas inferiores e 2 foram absolutamente negativos. Os que apresentaram pápulas inferiores a 5 mm foram submetidos ao BCG, recebendo 0,20 g de vacina, de uma a três vêzes, com 7 dias de intervalo. Notaram quase iniformemente aumento de diámetro das pápulas pela ação da vacina, enquanto que nos não calmetizados houve diminuição...(AU)^ipt.
Descriptors:Mycobacterium leprae/citol
Mycobacterium leprae/genet
Mycobacterium tuberculosis/citol
Mycobacterium tuberculosis/genet
Antígeno de Mitsuda/genet
Antígeno de Mitsuda/imunol
Vacina BCG/imunol
Electronic Medium:http://hansen.bvs.ilsl.br/textoc/revistas/1952/PDF/v20n1/v20n1a01.pdf / pt
Location:BR191.1


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Id:18842
Author:Azulay, R. D; Neves, R. G; Azulay, J. D.
Title:A reação lepromínica em cobaios após prévia inoculação do BCG e de Mycobacterium tuberculosis morto por irradiação.
Source:Rev. Bras. Leprol;27(2):81-86, abr.-jun. 1959. ^btab.
Abstract:Os autores estudaram nessa experiência a capacidade dos cobaios se tornarem lepromino-positivos; após a inoculação da vacina Parke Davis (Mycobacterium tuberculosis morto por irradiação) ou do BCG. I)- Cobaios inoculados subcutâneamente com a vacina Parke Davis (1 dose de 0,5 ml., semanalmente, durante 3 semanas) e testados com a lepromina integral mostraram os seguintes resultados: 80% de negatividade 3 20% de reações duvidosas. II)- Cobaios inoculados com BCG., por via oral, testados com lepromina integral, forneceram percentuais de positividade variáveis com as doses: a) com 60 mg. de BCG., por kg. de peso, os resultados foram: 47,0% reações positivas; 41,2% reações duvidosas; 11,8% negativas. b) com 120 mg. de BCG. por kg. de peso, os resultados foram: 25,0% reações positivas; 33,3% reações duvidosas; 41,7% reações negativas. c) o grupo contrôle fornecau os seguintes resultados: 12,5% reações duvidosas; 87,5% reações negativas. Os resultados desta experiência mostram: I)- A vacina Parke Davis preparada com Mycobacterium tuberculosis mortos por irradiação não induz positividade leprom¡nica, no cobaio. II)- O BCG administrado oralmente ‚ capaz de provocar a positividade lepromínica, no cobaio. III)- A julgar pelos resultados de experiência anteriores dos autores mostrando que a dose de 30 mg. de BCG por kg. de peso provocou, no cobaio, a mais elevada positividade lepromínica (87,5%) e doses maiores e menores do que 30 mg. (experiência anterior e atual) forneceram percentuais mais baixos de positividade, os autores pensam que aquela dose deve ser a ideal. IV- É interessante referir que a dose de 30 mg. por kg. de peso ‚ muito próxima da usada no Brasil (28 mg. por kg. de peso) para a premunição de recem-nascidos. (AU)^ipt.
Descriptors:Hanseníase/quimioter
Hanseníase/imunol
Hanseníase/fisiopatol
Hanseníase/reabil
Mycobacterium bovis/imunol
Mycobacterium bovis/fisiol
Mycobacterium tuberculosis/citol
Mycobacterium tuberculosis/fisiol
Limits:Humanos
Masculino
Feminino
Electronic Medium:http://hansen.bvs.ilsl.br/textoc/revistas/brasleprol/1959/PDF/v27n2/v27n2a02.pdf / pt
Location:BR191.1


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Id:18614
Author:Hadler, W. A; Ziti, L. M.
Title:Estudo da reação da lepromina no rato prèviamente inoculado co M. lepraemurium e com M. tuberculosis (BCG) / Study of the lepromin reaction in rats previously inoculated with M. lepraemurium and M. tuberculosis (BCG)
Source:Rev. bras. Leprol;23(1/4):53-75, jan.-dec. 1955. ^bilus, ^btab, ^bgraf.
Abstract:The influence of previous inoculation of mycobacteria on the results of lepromin reactions is studied in rats. This animal, when normal always shows a macroscopically and histological negative lepromin reaction. A comparative study was made between normal, adult rats (controls) and rats, previously injected by the intra-peritoneal route with about 5 mg of M. lepraemurium or 5,5 mg of M. tuberculosis (BCG strain)...(AU)^ien.
Descriptors:Antígeno de Mitsuda/bios
Antígeno de Mitsuda/quim
Antígeno de Mitsuda/imunol
Antígeno de Mitsuda/fisiol
Mycobacterium lepraemurium/ef drogas
Mycobacterium lepraemurium/fisiol
Mycobacterium tuberculosis/ef drogas
Mycobacterium tuberculosis/fisiol
Limits:Animais
Electronic Medium:http://hansen.bvs.ilsl.br/textoc/revistas/brasleprol/1955/PDF/v23n1-4a04.pdf / pt
Location:BR191.1


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Id:18591
Author:Hadler, W. A.
Title:Estudo comparado das lesões provocadas por suspensões de "M. leprae" e de "M. tuberculosis, injetadas por via intradêrmica, em cobaios préviamente vacinados pelo BCG / Comparative study of injuries caused by suspensions of "M. leprae" and of "M. tuberculosis, injected intradermally in guinea pigs previously vaccinated with BCG
Source:Rev. bras. Leprol;22(2):109-123, jun. 1954. ^btab.
Abstract:The lesions suspensions by intracutaneous injections of M. tuberculosis and M. leprosy suspensions, in BCG vaccinated guinea pigs, are cytologically alike; the cells of the inflmmatory tissue are morphologically and physiologically indicals and constitute lesions of a same type, with a central abscess surrounded by the tuberculosis inflammatory tissue. This cytological and histological identity makes easy the comparative study of the lesions by these two mycobacteria in vaccinated guinea pigs. In opposition in normal guinea pigs the same comparison is difficulted by the presence of the abscess observed only animals injected with M. tuberculosis. Ninety eight normal guinea pigs were innoculated with 33.0 mg of BCG, by peritoneal or intramuscular route. Fourt days after 58 guineas pigs were injected intracutaneously with 0,05 ml of a M. tuberculosis (BCG) suspension, containing 0.165 mg of bacilli; 22 guinea pigs were injected by this same via with 0, 1 ml of lepromin contaning nearly 0.074 mg of M. leprae; the other 20 guinea pigs were injected by this same route with 0.165 mg of BCG on a side and with 0,1 ml of lepromin containing 0.170 mg of M. leprae on the other side. The macroscopic and microscopic lesions suscitated by the intracutaneous injection of these two mycobacteria were observed during 50 days. The macroscopic examination shows: a) the reaction provoked by 0.165 mg of BCG is stronger but disappears at the 30th day after innoculation; b) the reaction suscitated by approximately 0.074 mg of M. leprae is weaker but vanishes only 40days after innoculation. The histological study of the lesions shows: a) the injection of 0.165 mg of BCG induces stronger reactions which have shorter evolutive course, with complete cicatrization of the 50th day; b) the injection of nearly 0.170 mg of M. leprae produces weaker lesions which have longer evolutive course, without complete cicatrization at the 50th day after innoculation. The evolutive speed of the... (AU)^ien.
Descriptors:Mycobacterium leprae/genet
Mycobacterium leprae/fisiol
Mycobacterium leprae/patogen
Mycobacterium tuberculosis/genet
Mycobacterium tuberculosis/fisiol
Mycobacterium tuberculosis/patogen
Pele/citol
Pele/les
Limits:Animais
Electronic Medium:http://hansen.bvs.ilsl.br/textoc/revistas/brasleprol/1954/PDF/v22n2/v22n2a01.pdf / pt
Location:BR191.1


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Id:18388
Author:Jamieson, S. E; Miller, E. N; Black, G. F; Peacock, C. S; Cordel, H. J; Howson, J. M. M; Shaw, M. A; Burgner, D; Xu, W; Lins-Lainson, Z; Shaw, J. J; Ramos, F; Silveira, F; Blackwell, J. M
Title:Evidence for cluster of genes on chromosome 17q11-q21 controlling susceptibility to tuberculosis and leprosy in Brazilians ..-
Source:s.l; s.n; 2004. 12 p. ilus, tab.
Abstract:The region of conserved synteny on mouse chromosome 11/human 17q11-q21 is known to carry a susceptibility gene(s) for intramacrophage pathogens. The region is rich in candidates including NOS2A, CCL2/MCP-1, CCL3/MIP-1x, CCL4/mip-1b, CCL5/RANTES, CCR7, STAT3 and STAT5A/5B. To examine the region in man, we studied 92 multicase tuberculosis (627 individuals) and 72 multicase leprosy (372 individuals) families from Brazil. Multipoint nonparametric analysis (ALLEGRO) using 16 microsatallites shows two peaks of linkage for leprosy at D17S250 (Z score 2.34; P=0.01) and D17S1795 (Z 2.67; P=0.004) and a single peak for tuberculosis at D17S250 (Z 2.04; P=0.02). Combined analysis shows significant linkage genes contribute, 49 informative single nucleotide polymorphisms were typed in candidate genes. Family-based allelic associationtesting that was robust to family clustering demonstrated siginificant associations with tuberculosis susceptibility at four loci separated by intervals (NOS2A-8.4Mb-CCL18-32.3kb-CCL4-6.04 Mb-STAT5B) up to several Mb. Stepwise conditional logistic regression analysis using a case/pseudo-control data set showed that the four genes contributed separate main effects, consistent with a cluster of susceptibility genes across 17q11.2 (AU).
Descriptors:CROMOSSOMOS HUMANOS PAR 17/genet
CROMOSSOMOS HUMANOS PAR 17/imunol
PREDISPOSICAO GENETICA PARA DOENÇA
MYCOBACTERIUM LEPRAE/imunol
MYCOBACTERIUM LEPRAE/metab
MYCOBACTERIUM TUBERCULOSIS/genet
MYCOBACTERIUM TUBERCULOSIS/imunol
MYCOBACTERIUM TUBERCULOSIS/metab
HANSENIASE/genet
 TUBERCULOSE/genet
 BRASIL/etnol
 BRASIL/epidemiol
Limits:HUMANO
ANIMAL
Location:BR191.1; 09314/s


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Id:18387
Author:Miller, E. N; Jamieson, S. E; Jobert, C; Fakiola, M; Peacock, C. S; Cordell, H. J; Shaw, M. A; Lins-Lainson, Z; Shaw, J. J; Ramos, F; Silveira, F; Blackwell, J. M
Title:Genome-wide scans for leprosy and tuberculosis susceptibility genes in Brazilians ..-
Source:s.l; s.n; 2004. 5 p. tab, graf.
Abstract:Genome-wide scans were conducted for tuberculosis and leprosy per se in Brazil. At stage 1,405 markers (10 cM map) were typed in 16 (178 individuals) tuberculosis and 21 (173 individuals) leprosy families. Nonparametric multipoint analysis detected 8 and 9 chromosomal regions respectively with provisional evidence (P<0.05) for linkage. A stage 2, 58 markers from positive regions were typed in a second set of 22 (176 individuals) tuberculosis families, with 22 additional markers types in all families; 42 positive markers in 50 (192 individuals) new leprosy families, and 30 additional markers in all families. Three regions (10q26.13, 11q12.3, 20p12.1) retained suggestive evidence (peak LOD scores 1.31, 1.78, 1.78; P=0.007, 0.0018, 0.0021) for linkage to tuberculosis, 3 regions (6p21.32, 17q22, 20p13) to leprosy (HLA-DQA, 3.23, P=5.8 x 10-5; D17S1868.2.38, P=0.0005; D20S889, 1.51, P=0.004). The peak at D20S889 for leprosy is 3.5 Mb distal to that reported at D20S115 for leprosy in India (AU).
Descriptors:PREDISPOSICAO GENETICA PARA DOENÇA
MYCOBACTERIUM LEPRAE
MYCOBACTERIUM TUBERCULOSIS
HANSENIASE/genet
TUBERCULOSE/genet
MYCOBACTERIUM LEPRAE/imunol
 MYCOBACTERIUM LEPRAE/metab
 MYCOBACTERIUM TUBERCULOSIS/imunol
 MYCOBACTERIUM TUBERCULOSIS/metab
Location:BR191.1; 09313/s


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Id:18234
Author:Das, Pranab K; Rambukkan, Anura; Baas, J. G; Groothuuis, Dick G; Halperin, Moshe
Title:Enzyme-linked immunosorbent assay for distinguishing serological responses of lepromatous and tuberculoid leprosies to the 29/33-kilodalton doublet and 64-kilodalton antigens of Mycobacterium tuberculosis ..-
Source:s.l; s.n; Feb. 1990. 4 p. ilus, tab.
Abstract:Immunoblot assays for the antibodies to Mycobacterium tuberculosis sonic extracts showed that all serum specimens of 40 lepromatous and 28 tuberculoid leprosy patient reacted in a significant amnner to 29/33-kilodalton (kDa) doublet and 64-kDa antigens, respectively. By using an enzyme-linked immunosorbent assay, we observed a significantly high immunoglobulin G antibody titer to the purified M. tuberculosis 29/33-kDa doublet and 64-kDa antigens in lepromatous and tuberculoid leprosy pateints, respectively, as compared with normal subjects and tuberculosis patients. This enzyme-linked immunosorbent assay serology may be useful for distinguihing two polar types of leprosy and for diagnosing leprosy in general (AU).
Descriptors:HANSENIASE VIRCHOWIANA/imunol
HANSENIASE TUBERCULOIDE/imunol
MYCOBACTERIUM TUBERCULOSIS/imunol
ELISA/util
IMUNOGLOBULINA G/imunol
IMUNOGLOBULINA G/ultraest
HANSENIASE/imunol
 MICOBACTERIOSE/imunol
Limits:HUMANO
Location:BR191.1; 09295/s


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Id:18203
Author:Anon
Title:The US-Japan Cooperative Medical Science Program Tuberculosis and leprosy Panel's 36th Annual Research Conference, New Orleans, Louisiana, USA, 15-17 July 2001 ..-
Source:s.l; s.n; 2002. 23 p. .
Conference:Present in: Leprosy Panel annual research conference, 36, New Orleans, 15-17 july 2001.
Descriptors:MYCOBACTERIUM/clas
MYCOBACTERIUM/cresc
MYCOBACTERIUM/imunol
MYCOBACTERIUM/patogen
MICOBACTERIAS ATIPICAS/cresc
MICOBACTERIAS ATIPICAS/imunol
MICOBACTERIAS ATIPICAS/isol
MICOBACTERIAS ATIPICAS/metab
MICOBACTERIAS ATIPICAS/fisiol
MICOBACTERIAS ATIPICAS/patogen
MYCOBACTERIUM AVIUM/cresc
MYCOBACTERIUM AVIUM/imunol
MYCOBACTERIUM AVIUM/patogen
MYCOBACTERIUM BOVIS/imunol
MYCOBACTERIUM BOVIS/patogen
MYCOBACTERIUM LEPRAE/imunol
MYCOBACTERIUM LEPRAE/patogen
MYCOBACTERIUM TUBERCULOSIS/cresc
MYCOBACTERIUM TUBERCULOSIS/imunol
MYCOBACTERIUM TUBERCULOSIS/isol
MYCOBACTERIUM TUBERCULOSIS/patogen
ANTITUBERCULOSOS/admin
ANTITUBERCULOSOS/ef adv
ANTITUBERCULOSOS/uso terap
COOPERACAO INTERNACIONAL
 COOPERACAO TECNICA
 VACINA BCG/admin
Limits:HUMANO
ANIMAL
Location:BR191.1; 09068/s


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Id:18000
Author:Silva, Célio Lopes.
Title:Estudo sobre a atividade protetora da proteína de choque térmico HSP65 nas infecções micobacterianas / Study on the protective activity of the protein of thermal shock HSP65 in the micobacterianas infections
Source:Hansen. int;(n.esp):121-128, Jun. 1998. .
Descriptors:MYCOBACTERIUM TUBERCULOSIS/quim
MYCOBACTERIUM TUBERCULOSIS/citol
MYCOBACTERIUM TUBERCULOSIS/genet
MYCOBACTERIUM TUBERCULOSIS/imunol
MYCOBACTERIUM TUBERCULOSIS/metab
MYCOBACTERIUM TUBERCULOSIS/fisiol
VACINA BCG/genet
VACINA BCG/imunol
VACINA BCG/farmacol
INFECCOES BACTERIANAS/compl
 INFECCOES BACTERIANAS/diag
 INFECCOES BACTERIANAS/genet
 INFECCOES BACTERIANAS/imunol
 INFECCOES BACTERIANAS/fisiopatol
Limits:RELATO DE CASO
ESTUDO COMPARATIVO
HUMANO
Location:BR191.1


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Id:17022
Author:Feldman, William H; Moses, Harold E.
Title:The effect of diphtheria toxoid on experimental tuberculosis.
Source:Int. J. Lepr;11(n.esp):36-42, Dec. 1943. tab.
Abstract:Using guinea-pigs infected experimentally with human tubercle bacilli and rabbits infected experimentally with bovine tubercle bacilli, a series of studies was made to ascertain whether or not diphtheria toxoid would influence the expected course of the resultant tuberculosis. Seventhy guinea-pigs and ten rabbits were used. The administration of toxoid in relation to the inoculation of the animals with the infective agent varied in the different studies. In two groups of guinea-pigs, treatment was started the same day that the animals were infected. In another group treatment was delayed for four weeks after the animals had been infected, and in another group the guinea-pigs had received two doses of toxoid one month apart before being infected. The rabbits received the first dose of toxoid simultaneously with the infective organism. The initial dose of toxoid for the guinea-pigs was 0.1 cc. Subsequent doses were administered every two weeks, with each succeeding dose 0.05cc. greater than the preceding dose. The initial dose for rabbits was 0.2cc. This was increased by 0.1cc. at each succeeding injection, whch was at two week intervals. The toxoid was injected intramuscularly. The experiments continued until all of the animals had died, which was somewhat in excess of seven months. All of the animals died of tuberculosis, and there were no significant differences between the treated and the untreated froups. The results indicate quite convincingly that under the conditions of the experiments diphtheria toxoid failed to exert any significant deterrent effect on tuberculosis experimentally induced in guine´-pigs and in rabbits. (AU).
Descriptors:TUBERCULOSE
MYCOBACTERIUM TUBERCULOSIS/ef drogas
MYCOBACTERIUM TUBERCULOSIS/isol
MYCOBACTERIUM TUBERCULOSIS/patogen
TOXOIDE DIFTERICO/farmacol
TOXOIDE DIFTERICO/uso terap
MYCOBACTERIUM LEPRAE/metab
 MYCOBACTERIUM LEPRAE/patogen
Limits:ANIMAL
Location:BR191.1


  14 / 89 HANSEN  
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Id:13965
Author:Hoal, Eileen G
Title:Human genetic susceptibility to tuberculosis and other mycobacterial diseases ..-
Source:s.l; s.n; 2002. 5 p. .
Abstract:The existence of a genetic component in mycobacterial disease susceptibility is no longer in doubt and the investigations now being conducted aim to determine which genes are involved, to what extent, and in which disease phenotype they are relevant. In certain rare instances of susceptibility to poorly pathogenic mycobacteria, the genetic component is clear. The approaches employed to elucidate common disease susceptibility include linkage studies, particularly genome-wide linkage analysis of both tuberculosis and leprosy, and association studies. A number of candidate genes have shown association with tuberculosis, and in many cases, on replication of the study, association has been confirmed in a disparate population, indicating the wider importance of the gene in the disease process. In other instances, associations appear to be particular to a population or a subtype of disease. (AU).
Descriptors:PREDISPOSICAO GENETICA PARA DOENÇA
LIGACAO (GENETICA)
GENOTIPO
MYCOBACTERIUM TUBERCULOSIS/patogen
TUBERCULOSE/epidemiol
TUBERCULOSE/genet
FENOTIPO
Limits:HUMANO
SUPPORT, NON-U.S. GOV'T
Electronic Medium:http://www.ilsl.br
Location:BR191.1; 09058/s


  15 / 89 HANSEN  
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Id:13944
Author:Bochud, Pierre-Yves; Hawn, Thomas R; Aderem, Alan
Title:Cutting edge: a toll-like receptor 2 polymorphism that is associated with lepromatous leprosy is unable to mediate mycobacterial signaling ..-
Source:s.l; s.n; 2003. 4 p. graf.
Abstract:Toll-like receptors (TLRs) are key mediators of the innate immune response to microbial pathogens. We investigated the role of TLRs in the recognition of Mycobacterium leprae and the significance of TLR2Arg(677)Trp, a recently discovered human polymorphism that is associated with lepromatous leprosy. In mice, TNF-alpha production in response to M. leprae was essentially absent in TLR2-deficient macrophages. Similarly, human TLR2 mediated M. leprae-dependent activation of NF-kappaB in transfected Chinese hamster ovary and human embryonic kidney 293 cells, with enhancement of this signaling in the presence of CD14. In contrast, activation of NF-kappaB by human TLR2Arg(677)Trp was abolished in response to M. leprae and Mycobacterium tuberculosis. The impaired function of this TLR2 variant provides a molecular mechanism for the poor cellular immune response associated with lepromatous leprosy and may have important implications for understanding the pathogenesis of other mycobacterial infections. (AU).
Descriptors:CELULAS CHO
LINHAGEM CELULAR
IMUNIDADE NATURAL/genet
ARGININA/genet
HANSENIASE VIRCHOWIANA/genet
HANSENIASE VIRCHOWIANA/imunol
HANSENIASE VIRCHOWIANA/microbiol
GLICOPROTEINAS DE MEMBRANA/defic
GLICOPROTEINAS DE MEMBRANA/genet
GLICOPROTEINAS DE MEMBRANA/fisiol
CAMUNDONGOS ENDOGÂMICOS C57BL
CAMUNDONGOS KNOCKOUT
MYCOBACTERIUM LEPRAE/imunol
MYCOBACTERIUM TUBERCULOSIS/imunol
POLIMORFISMO DE UM UNICO NUCLEOTIDIO/imunol
TRANSDUCAO DE SINAL/imunol
TRIPTOFANO/genet
RECEPTORES DA SUPERFICIE CELULAR/defic
RECEPTORES DA SUPERFICIE CELULAR/genet
RECEPTORES DA SUPERFICIE CELULAR/fisiol
Limits:HUMANO
ANIMAL
HAMSTERS
CAMUNDONGOS
SUPPORT, NON-U.S. GOV'T
SUPPORT, U.S. GOV'T, P.H.S.
Electronic Medium:http://www.ilsl.br
Location:BR191.1; 09085/s


  16 / 89 HANSEN  
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Id:13666
Author:Bala, Lakshmi; Anand, Sukumar; Sinha, Sudhir
Title:Enhancement of human T cell response to a peptide epitope of 38kDa antigen of Mycobacterium tuberculosis by liposomes ..-
Source:s.l; s.n; 2002. 9 p. graf.
Abstract:Diagnosis of tuberculosis a problem, specially in the regions harboring an abundance of both pathogenic and non-pathogenic mycobacteria. This study was undertaken to assess in such a situation the predictive value of proliferative T cell response to a peptide epitope ('38G') of the 38 kDa membrane protein of Mycobacterium tuberculosis. 3[H]-thymidine incorporation assays were done with peripheral blood mononuclear cells of tuberculoid leprosy and pulmonary tuberculosis patients. The donors were also classified as PPD responders (Stimulation Index, SI> 3) or non-responders (SI < or = 3) on the basis of their T cell response to the 'Purified Protein Derivative (PPD)' of M. tuberculosis. 38G peptide was used in either free or liposome-associated form prepared by the technique of 'Dehydration-rehydration Vesicles' (Kirby and Gregoriadis, 1984). While free peptide failed to induce a positive response in study subjects, its liposomal form was T cell stimulatory and distinguished, to certain extent, between PPD responders (corresponding SI > 3 in 54 per cent subjects) and non-responders (SI > 3 in 29 per cent subjects). However, it did not differentiate between leprosy and tuberculosis. The study supports use of liposomes as adjuvant vehicles for antigenic peptides designed to activate human T cells. (AU).
Descriptors:ANTIGENOS DE BACTERIAS/admin
ANTIGENOS DE BACTERIAS/quim
ANTIGENOS DE BACTERIAS/genet
HANSENIASE TUBERCULOIDE/diag
HANSENIASE TUBERCULOIDE/imunol
EPITOPOS/admin
EPITOPOS/quim
EPITOPOS/genet
DIAGNOSTICO DIFERENCIAL
MYCOBACTERIUM TUBERCULOSIS/quim
MYCOBACTERIUM TUBERCULOSIS/genet
MYCOBACTERIUM TUBERCULOSIS/imunol
DADOS DE SEQUÊNCIA MOLECULAR
SEQUÊNCIA DE AMINOACIDOS
LINFOCITOS T/imunol
TESTE TUBERCULINICO
TUBERCULOSE PULMONAR/diag
TUBERCULOSE PULMONAR/imunol
TRANSFORMACAO LINFOCITICA
LIPOSSOMOS
 PESO MOLECULAR
Limits:ESTUDO COMPARATIVO
HUMANO
IN VITRO
SUPPORT, NON-U.S. GOV'T
Electronic Medium:http://www.ilsl.br
Location:BR191.1; 09061/s


  17 / 89 HANSEN  
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Id:13658
Author:Sassetti, Christopher M; Boyd, Dana H; Rubin, Eric J
Title:Genes required for mycobacterial growth defined by high density mutagenesis ..-
Source:s.l; s.n; 2003. 8 p. tab, graf.
Abstract:Despite over a century of research, tuberculosis remains a leading cause of infectious death worldwide. Faced with increasing rates of drug resistance, the identification of genes that are required for the growth of this organism should provide new targets for the design of antimycobacterial agents. Here, we describe the use of transposon site hybridization (TraSH) to comprehensively identify the genes required by the causative agent, Mycobacterium tuberculosis, for optimal growth. These genes include those that can be assigned to essential pathways as well as many of unknown function. The genes important for the growth of M. tuberculosis are largely conserved in the degenerate genome of the leprosy bacillus, Mycobacterium leprae, indicating that non-essential functions have been selectively lost since this bacterium diverged from other mycobacteria. In contrast, a surprisingly high proportion of these genes lack identifiable orthologues in other bacteria, suggesting that the minimal gene set required for survival varies greatly between organisms with different evolutionary histories. (AU).
Descriptors:ELEMENTOS DE DNA TRANSPONIVEIS
GENES BACTERIANOS
MUTAGÊNESE
MYCOBACTERIUM LEPRAE/cresc
MYCOBACTERIUM LEPRAE/genet
MYCOBACTERIUM TUBERCULOSIS/cresc
MYCOBACTERIUM TUBERCULOSIS/genet
EVOLUCAO MOLECULAR
Limits:SUPPORT, NON-U.S. GOV'T
SUPPORT, U.S. GOV'T, P.H.S.
Electronic Medium:http://www.ilsl.br
Location:BR191.1; 09138/s


  18 / 89 HANSEN  
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Id:13656
Author:Bleharski, Joshua R; Li, Huiying; Meinken, Christoph; Graeber, Thomas G; Ochoa, Maria-Teresa; Yamamura, Masahiro; Burdick, Anne; Sarno, Euzenir N; Wagner, Manfred; Röllinghoff, Martin; Rea, Thomas H; Colonna, Marco; Stenger, Steffen; Bloom, Barry R; Eisenberg, David; Modlin, Robert L
Title:Use of genetic profiling in leprosy to discriminate clinical forms of the disease ..-
Source:s.l; s.n; Sep. 2003. 4 p. graf.
Abstract:Leprosy presents as a clinical and immunological spectrum of disease. With the use of gene expression profiling, we observed that a distinction in gene expression correlates with and accurately classifies the clinical form of the disease. Genes belonging to the leukocyte immunoglobulin-like receptor (LIR) family were significantly up-regulated in lesions of lepromatous patients suffering from the disseminated form of the infection. In functional studies, LIR-7 suppressed innate host defense mechanisms by shifting monocyte production from interleukin-12 toward interleukin-10 and by blocking antimicrobial activity triggered by Toll-like receptors. Gene expression profiles may be useful in defining clinical forms of disease and providing insights into the regulation of immune responses to pathogens. (AU).
Descriptors:HANSENIASE VIRCHOWIANA/clas
HANSENIASE VIRCHOWIANA/genet
HANSENIASE VIRCHOWIANA/imunol
HANSENIASE VIRCHOWIANA/fisiopatol
HANSENIASE TUBERCULOIDE/clas
HANSENIASE TUBERCULOIDE/genet
HANSENIASE TUBERCULOIDE/imunol
HANSENIASE TUBERCULOIDE/fisiopatol
CITOCINAS/genet
CITOCINAS/metab
PERFILACAO DA EXPRESSÃO GÊNICA
ANALISE POR CONGLOMERADOS
CONTAGEM DE COLÔNIA MICROBIANA
REGULACAO DA EXPRESSÃO GÊNICA
IMUNIDADE CELULAR
IMUNIDADE NATURAL
MACROFAGOS ALVEOLARES/microbiol
GLICOPROTEINAS DE MEMBRANA/imunol
MYCOBACTERIUM TUBERCULOSIS/cresc
MYCOBACTERIUM TUBERCULOSIS/imunol
REACAO EM CADEIA DA POLIMERASE
RECEPTORES IMUNOLOGICOS/genet
RECEPTORES IMUNOLOGICOS/metab
RECEPTORES DA SUPERFICIE CELULAR/imunol
REGULACAO PARA CIMA
 Análise de Componente Principal
 ALGORITMOS
 GENES DE IMUNOGLOBULINAS
 ANALISE DE SEQUÊNCIA COM SERIES DE OLIGONUCLEOTIDIOS
Limits:SUPPORT, NON-U.S. GOV'T
HUMANO
SUPPORT, U.S. GOV'T, P.H.S.
Electronic Medium:http://www.ilsl.br
Location:BR191.1; 09142/s; BR191.1; 09145/s


  19 / 89 HANSEN  
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Id:13635
Author:Hoal, Eileen G
Title:Human genetic susceptibility to tuberculosis and other mycobacterial diseases ..-
Source:s.l; s.n; 2002. 5 p. .
Abstract:The existence of a genetic component in mycobacterial disease susceptibility is no longer in doubt and the investigations now being conducted aim to determine which genes are involved, to what extent, and in which disease phenotype they are relevant. In certain rare instances of susceptibility to poorly pathogenic mycobacteria, the genetic component is clear. The approaches employed to elucidate common disease susceptibility include linkage studies, particularly genome-wide linkage analysis of both tuberculosis and leprosy, and association studies. A number of candidate genes have shown association with tuberculosis, and in many cases, on replication of the study, association has been confirmed in a disparate population, indicating the wider importance of the gene in the disease process. In other instances, associations appear to be particular to a population or a subtype of disease.(AU).
Descriptors:PREDISPOSICAO GENETICA PARA DOENÇA
GENOTIPO
LIGACAO (GENETICA)
MYCOBACTERIUM TUBERCULOSIS/patogen
FENOTIPO
TUBERCULOSE/epidemiol
TUBERCULOSE/genet
Limits:HUMANO
SUPPORT, NON-U.S. GOV'T
Electronic Medium:http://www.ilsl.br
Location:BR191.1; 09044/s


  20 / 89 HANSEN  
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Id:13632
Author:Abate, Getahun
Title:Drug-resistant tuberculosis in Ethiopia: problem scenarios and recommendation ..-
Source:s.l; s.n; 2002. 8 p. tab.
Abstract:Tuberculosis (TB) is a major public health problem in Ethiopia. This review is prepared to indicate possible future challenges related to tuberculosis control and it includes previous reports of drug-resistant surveys in Ethiopia. Drug-resistant TB, both initial and acquired, was reported from different regions of the country. In studies from 1984 to 2001, the initial resistance to isoniazid ranges from 2 per cent to 21 per cent and initial resistance to streptomycin ranges from 2 to 20 per cent. Multidrug-resistance (MDR) TB defined as resistance to at least isoniazid and rifampicin was also reported in about 1.2 per cent of new cases and 12 per cent of re-treatment cases. In all studies which included ethambutol susceptibility test, ethambutol resistance is either nil or very low (below 0.5 per cent). All MDR isolates were susceptible to ethambutol. Treatment and re-treatment regimens recommended by the National TB/Leprosy Control Program could be effective on all cases other than those with MDR-TB. MDR-TB is difficult to cure. Therefore, special emphasis should be given to control the spread of MDR-TB. Lack of control efforts may lead to the increased resistance to both first- and second-line drugs. A well supported and controlled special treatment unit, which uses both first-line and second-line drugs is required for a proper management of these cases and for effective control of the spread of MDR-TB. A uniform susceptibility to ethambutol can be taken as an advantage to develop standard low-cost re-treatment regimen for MDR-TB patients. (AU).
Descriptors:ANTITUBERCULOSOS/uso terap
ETIOPIA/epidemiol
MYCOBACTERIUM TUBERCULOSIS/ef drogas
TUBERCULOSE RESISTENTE A MULTIPLAS DROGAS/quimioter
TUBERCULOSE RESISTENTE A MULTIPLAS DROGAS/epidemiol
Limits:HUMANO
Electronic Medium:http://www.ilsl.br
Location:BR191.1; 09041/s



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